Investigation of the mechanism of action of Porana sinensis Hemsl. against gout arthritis using network pharmacology and experimental validation.
Identifieur interne : 000127 ( Main/Exploration ); précédent : 000126; suivant : 000128Investigation of the mechanism of action of Porana sinensis Hemsl. against gout arthritis using network pharmacology and experimental validation.
Auteurs : Xia Du [République populaire de Chine] ; Lintao Zhao [République populaire de Chine] ; Yuanyuan Yang [République populaire de Chine] ; Zijia Zhang [République populaire de Chine] ; Jing Hu [République populaire de Chine] ; Hui Ren [République populaire de Chine] ; Zhiyong Chen [République populaire de Chine] ; Ye Li [République populaire de Chine]Source :
- Journal of ethnopharmacology [ 1872-7573 ] ; 2020.
Descripteurs français
- KwdFr :
- Acide chlorogénique (pharmacologie), Acide chlorogénique (usage thérapeutique), Animaux (MeSH), Articulation talocrurale (anatomopathologie), Articulation talocrurale (effets des médicaments et des substances chimiques), Cartes d'interactions protéiques (MeSH), Convolvulaceae (MeSH), Coumarines (pharmacologie), Coumarines (usage thérapeutique), Cytokines (sang), Extraits de plantes (pharmacologie), Extraits de plantes (usage thérapeutique), Facteur de différenciation myéloïde-88 (génétique), Goutte articulaire (anatomopathologie), Goutte articulaire (génétique), Goutte articulaire (métabolisme), Goutte articulaire (traitement médicamenteux), Mâle (MeSH), Pharmacologie (méthodes), Rat Sprague-Dawley (MeSH), Récepteur de type Toll-2 (génétique), Simulation de docking moléculaire (MeSH).
- MESH :
- anatomopathologie : Articulation talocrurale, Goutte articulaire.
- effets des médicaments et des substances chimiques : Articulation talocrurale.
- génétique : Facteur de différenciation myéloïde-88, Goutte articulaire, Récepteur de type Toll-2.
- métabolisme : Goutte articulaire.
- méthodes : Pharmacologie.
- pharmacologie : Acide chlorogénique, Coumarines, Extraits de plantes.
- sang : Cytokines.
- traitement médicamenteux : Goutte articulaire.
- usage thérapeutique : Acide chlorogénique, Coumarines, Extraits de plantes.
- Animaux, Cartes d'interactions protéiques, Convolvulaceae, Mâle, Rat Sprague-Dawley, Simulation de docking moléculaire.
English descriptors
- KwdEn :
- Animals (MeSH), Ankle Joint (drug effects), Ankle Joint (pathology), Arthritis, Gouty (drug therapy), Arthritis, Gouty (genetics), Arthritis, Gouty (metabolism), Arthritis, Gouty (pathology), Chlorogenic Acid (pharmacology), Chlorogenic Acid (therapeutic use), Convolvulaceae (MeSH), Coumarins (pharmacology), Coumarins (therapeutic use), Cytokines (blood), Male (MeSH), Molecular Docking Simulation (MeSH), Myeloid Differentiation Factor 88 (genetics), Pharmacology (methods), Plant Extracts (pharmacology), Plant Extracts (therapeutic use), Protein Interaction Maps (MeSH), Rats, Sprague-Dawley (MeSH), Toll-Like Receptor 2 (genetics).
- MESH :
- chemical , blood : Cytokines.
- chemical , genetics : Myeloid Differentiation Factor 88, Toll-Like Receptor 2.
- chemical , pharmacology : Chlorogenic Acid, Coumarins, Plant Extracts.
- drug effects : Ankle Joint.
- drug therapy : Arthritis, Gouty.
- genetics : Arthritis, Gouty.
- metabolism : Arthritis, Gouty.
- methods : Pharmacology.
- pathology : Ankle Joint, Arthritis, Gouty.
- chemical , therapeutic use : Chlorogenic Acid, Coumarins, Plant Extracts.
- Animals, Convolvulaceae, Male, Molecular Docking Simulation, Protein Interaction Maps, Rats, Sprague-Dawley.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE
Porana sinensis Hemsl. has been widely used to treat joint pain and rheumatoid arthritis in traditional Chinese medicine (TCM). Although evidence exists to support a pharmacological action of P. sinensis for the treatment of gout arthritis (GA), the underlying mechanism of action remains unknown due to it being a multi-component and multi-target agent.
AIM OF THE STUDY
To clarify the active compounds and mechanism of P. sinensis against GA.
MATERIALS AND METHODS
The present study combined network pharmacology with experiments to clarify the mechanism of P. sinensis against GA. A protein-protein interaction network for gout was constructed to identify the potential drug targets, and molecular docking was subsequently performed to determine whether the protein was a target for the compounds of P. sinensis. KEGG pathway analysis was then conducted to elucidate the pathway involved in the P. sinensis-mediated treatment of gout. A rat model of GA was used to further investigate the mechanism of P. sinensis against GA.
RESULTS
The network pharmacology study indicates that coumarins and chlorogenic acids of P. sinensis may serve as additives to GA treatment. P. sinensis played a role in the treatment of GA by regulating the PI3K-Akt, MAPK, NF-kappa B and toll-like receptor pathways and so on. Moreover, experimental validation suggests that P. sinensis extract significantly suppressed the expression of TLR2 and MyD88 mRNA, regulating the release of cytokines (IL-1β, IL-4 and TGF-β), lowering lipid peroxidation (MDA) and increasing antioxidant status (SOD).
CONCLUSION
The present study clarifies the mechanism of P. sinensis against GA, and provides evidence to support its clinical use.
DOI: 10.1016/j.jep.2020.112606
PubMed: 31988013
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Investigation of the mechanism of action of Porana sinensis Hemsl. against gout arthritis using network pharmacology and experimental validation.</title><author><name sortKey="Du, Xia" sort="Du, Xia" uniqKey="Du X" first="Xia" last="Du">Xia Du</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Lintao" sort="Zhao, Lintao" uniqKey="Zhao L" first="Lintao" last="Zhao">Lintao Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yuanyuan" sort="Yang, Yuanyuan" uniqKey="Yang Y" first="Yuanyuan" last="Yang">Yuanyuan Yang</name><affiliation wicri:level="1"><nlm:affiliation>Xi'an Institute for Food and Drug Control, Xi'an, Shaanxi, 710054, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Xi'an Institute for Food and Drug Control, Xi'an, Shaanxi, 710054</wicri:regionArea><wicri:noRegion>710054</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Zijia" sort="Zhang, Zijia" uniqKey="Zhang Z" first="Zijia" last="Zhang">Zijia Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210</wicri:regionArea><wicri:noRegion>201210</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Jing" sort="Hu, Jing" uniqKey="Hu J" first="Jing" last="Hu">Jing Hu</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Zhiyong" sort="Chen, Zhiyong" uniqKey="Chen Z" first="Zhiyong" last="Chen">Zhiyong Chen</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address: chenzhiyong0612@sina.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address: 18829014325@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31988013</idno><idno type="pmid">31988013</idno><idno type="doi">10.1016/j.jep.2020.112606</idno><idno type="wicri:Area/Main/Corpus">000235</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000235</idno><idno type="wicri:Area/Main/Curation">000235</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000235</idno><idno type="wicri:Area/Main/Exploration">000235</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Investigation of the mechanism of action of Porana sinensis Hemsl. against gout arthritis using network pharmacology and experimental validation.</title><author><name sortKey="Du, Xia" sort="Du, Xia" uniqKey="Du X" first="Xia" last="Du">Xia Du</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Lintao" sort="Zhao, Lintao" uniqKey="Zhao L" first="Lintao" last="Zhao">Lintao Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yuanyuan" sort="Yang, Yuanyuan" uniqKey="Yang Y" first="Yuanyuan" last="Yang">Yuanyuan Yang</name><affiliation wicri:level="1"><nlm:affiliation>Xi'an Institute for Food and Drug Control, Xi'an, Shaanxi, 710054, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Xi'an Institute for Food and Drug Control, Xi'an, Shaanxi, 710054</wicri:regionArea><wicri:noRegion>710054</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Zijia" sort="Zhang, Zijia" uniqKey="Zhang Z" first="Zijia" last="Zhang">Zijia Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210</wicri:regionArea><wicri:noRegion>201210</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Jing" sort="Hu, Jing" uniqKey="Hu J" first="Jing" last="Hu">Jing Hu</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Zhiyong" sort="Chen, Zhiyong" uniqKey="Chen Z" first="Zhiyong" last="Chen">Zhiyong Chen</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address: chenzhiyong0612@sina.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author><author><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><affiliation wicri:level="1"><nlm:affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address: 18829014325@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003</wicri:regionArea><wicri:noRegion>710003</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of ethnopharmacology</title><idno type="eISSN">1872-7573</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Ankle Joint (drug effects)</term><term>Ankle Joint (pathology)</term><term>Arthritis, Gouty (drug therapy)</term><term>Arthritis, Gouty (genetics)</term><term>Arthritis, Gouty (metabolism)</term><term>Arthritis, Gouty (pathology)</term><term>Chlorogenic Acid (pharmacology)</term><term>Chlorogenic Acid (therapeutic use)</term><term>Convolvulaceae (MeSH)</term><term>Coumarins (pharmacology)</term><term>Coumarins (therapeutic use)</term><term>Cytokines (blood)</term><term>Male (MeSH)</term><term>Molecular Docking Simulation (MeSH)</term><term>Myeloid Differentiation Factor 88 (genetics)</term><term>Pharmacology (methods)</term><term>Plant Extracts (pharmacology)</term><term>Plant Extracts (therapeutic use)</term><term>Protein Interaction Maps (MeSH)</term><term>Rats, Sprague-Dawley (MeSH)</term><term>Toll-Like Receptor 2 (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide chlorogénique (pharmacologie)</term><term>Acide chlorogénique (usage thérapeutique)</term><term>Animaux (MeSH)</term><term>Articulation talocrurale (anatomopathologie)</term><term>Articulation talocrurale (effets des médicaments et des substances chimiques)</term><term>Cartes d'interactions protéiques (MeSH)</term><term>Convolvulaceae (MeSH)</term><term>Coumarines (pharmacologie)</term><term>Coumarines (usage thérapeutique)</term><term>Cytokines (sang)</term><term>Extraits de plantes (pharmacologie)</term><term>Extraits de plantes (usage thérapeutique)</term><term>Facteur de différenciation myéloïde-88 (génétique)</term><term>Goutte articulaire (anatomopathologie)</term><term>Goutte articulaire (génétique)</term><term>Goutte articulaire (métabolisme)</term><term>Goutte articulaire (traitement médicamenteux)</term><term>Mâle (MeSH)</term><term>Pharmacologie (méthodes)</term><term>Rat Sprague-Dawley (MeSH)</term><term>Récepteur de type Toll-2 (génétique)</term><term>Simulation de docking moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Myeloid Differentiation Factor 88</term><term>Toll-Like Receptor 2</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chlorogenic Acid</term><term>Coumarins</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Articulation talocrurale</term><term>Goutte articulaire</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Ankle Joint</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Arthritis, Gouty</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Articulation talocrurale</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arthritis, Gouty</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteur de différenciation myéloïde-88</term><term>Goutte articulaire</term><term>Récepteur de type Toll-2</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arthritis, Gouty</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pharmacology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Goutte articulaire</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Pharmacologie</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Ankle Joint</term><term>Arthritis, Gouty</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide chlorogénique</term><term>Coumarines</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Chlorogenic Acid</term><term>Coumarins</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Goutte articulaire</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Acide chlorogénique</term><term>Coumarines</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Convolvulaceae</term><term>Male</term><term>Molecular Docking Simulation</term><term>Protein Interaction Maps</term><term>Rats, Sprague-Dawley</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartes d'interactions protéiques</term><term>Convolvulaceae</term><term>Mâle</term><term>Rat Sprague-Dawley</term><term>Simulation de docking moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>ETHNOPHARMACOLOGICAL RELEVANCE</b></p><p>Porana sinensis Hemsl. has been widely used to treat joint pain and rheumatoid arthritis in traditional Chinese medicine (TCM). Although evidence exists to support a pharmacological action of P. sinensis for the treatment of gout arthritis (GA), the underlying mechanism of action remains unknown due to it being a multi-component and multi-target agent.</p></div><div type="abstract" xml:lang="en"><p><b>AIM OF THE STUDY</b></p><p>To clarify the active compounds and mechanism of P. sinensis against GA.</p></div><div type="abstract" xml:lang="en"><p><b>MATERIALS AND METHODS</b></p><p>The present study combined network pharmacology with experiments to clarify the mechanism of P. sinensis against GA. A protein-protein interaction network for gout was constructed to identify the potential drug targets, and molecular docking was subsequently performed to determine whether the protein was a target for the compounds of P. sinensis. KEGG pathway analysis was then conducted to elucidate the pathway involved in the P. sinensis-mediated treatment of gout. A rat model of GA was used to further investigate the mechanism of P. sinensis against GA.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The network pharmacology study indicates that coumarins and chlorogenic acids of P. sinensis may serve as additives to GA treatment. P. sinensis played a role in the treatment of GA by regulating the PI3K-Akt, MAPK, NF-kappa B and toll-like receptor pathways and so on. Moreover, experimental validation suggests that P. sinensis extract significantly suppressed the expression of TLR2 and MyD88 mRNA, regulating the release of cytokines (IL-1β, IL-4 and TGF-β), lowering lipid peroxidation (MDA) and increasing antioxidant status (SOD).</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The present study clarifies the mechanism of P. sinensis against GA, and provides evidence to support its clinical use.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31988013</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7573</ISSN><JournalIssue CitedMedium="Internet"><Volume>252</Volume><PubDate><Year>2020</Year><Month>Apr</Month><Day>24</Day></PubDate></JournalIssue><Title>Journal of ethnopharmacology</Title><ISOAbbreviation>J Ethnopharmacol</ISOAbbreviation></Journal><ArticleTitle>Investigation of the mechanism of action of Porana sinensis Hemsl. against gout arthritis using network pharmacology and experimental validation.</ArticleTitle><Pagination><MedlinePgn>112606</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0378-8741(19)34439-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jep.2020.112606</ELocationID><Abstract><AbstractText Label="ETHNOPHARMACOLOGICAL RELEVANCE" NlmCategory="BACKGROUND">Porana sinensis Hemsl. has been widely used to treat joint pain and rheumatoid arthritis in traditional Chinese medicine (TCM). Although evidence exists to support a pharmacological action of P. sinensis for the treatment of gout arthritis (GA), the underlying mechanism of action remains unknown due to it being a multi-component and multi-target agent.</AbstractText><AbstractText Label="AIM OF THE STUDY" NlmCategory="OBJECTIVE">To clarify the active compounds and mechanism of P. sinensis against GA.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">The present study combined network pharmacology with experiments to clarify the mechanism of P. sinensis against GA. A protein-protein interaction network for gout was constructed to identify the potential drug targets, and molecular docking was subsequently performed to determine whether the protein was a target for the compounds of P. sinensis. KEGG pathway analysis was then conducted to elucidate the pathway involved in the P. sinensis-mediated treatment of gout. A rat model of GA was used to further investigate the mechanism of P. sinensis against GA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The network pharmacology study indicates that coumarins and chlorogenic acids of P. sinensis may serve as additives to GA treatment. P. sinensis played a role in the treatment of GA by regulating the PI3K-Akt, MAPK, NF-kappa B and toll-like receptor pathways and so on. Moreover, experimental validation suggests that P. sinensis extract significantly suppressed the expression of TLR2 and MyD88 mRNA, regulating the release of cytokines (IL-1β, IL-4 and TGF-β), lowering lipid peroxidation (MDA) and increasing antioxidant status (SOD).</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The present study clarifies the mechanism of P. sinensis against GA, and provides evidence to support its clinical use.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Xia</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Lintao</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yuanyuan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Xi'an Institute for Food and Drug Control, Xi'an, Shaanxi, 710054, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zijia</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zhiyong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address: chenzhiyong0612@sina.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ye</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address: 18829014325@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>J Ethnopharmacol</MedlineTA><NlmUniqueID>7903310</NlmUniqueID><ISSNLinking>0378-8741</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003374">Coumarins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C507781">Myd88 protein, rat</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D053594">Myeloid Differentiation Factor 88</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C495343">Tlr2 protein, rat</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051195">Toll-Like Receptor 2</NameOfSubstance></Chemical><Chemical><RegistryNumber>318ADP12RI</RegistryNumber><NameOfSubstance UI="D002726">Chlorogenic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000843" MajorTopicYN="N">Ankle Joint</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015210" MajorTopicYN="N">Arthritis, Gouty</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002726" MajorTopicYN="N">Chlorogenic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName><QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027722" MajorTopicYN="Y">Convolvulaceae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003374" MajorTopicYN="N">Coumarins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName><QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053594" MajorTopicYN="N">Myeloid Differentiation Factor 88</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010600" MajorTopicYN="N">Pharmacology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName><QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060066" MajorTopicYN="N">Protein Interaction Maps</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017207" MajorTopicYN="N">Rats, Sprague-Dawley</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051195" MajorTopicYN="N">Toll-Like Receptor 2</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anti-inflammatory</Keyword><Keyword MajorTopicYN="N">Experimental validation</Keyword><Keyword MajorTopicYN="N">Gout arthritis</Keyword><Keyword MajorTopicYN="N">Network pharmacology</Keyword><Keyword MajorTopicYN="N">Porana sinensis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31988013</ArticleId><ArticleId IdType="pii">S0378-8741(19)34439-3</ArticleId><ArticleId IdType="doi">10.1016/j.jep.2020.112606</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Du, Xia" sort="Du, Xia" uniqKey="Du X" first="Xia" last="Du">Xia Du</name></noRegion><name sortKey="Chen, Zhiyong" sort="Chen, Zhiyong" uniqKey="Chen Z" first="Zhiyong" last="Chen">Zhiyong Chen</name><name sortKey="Hu, Jing" sort="Hu, Jing" uniqKey="Hu J" first="Jing" last="Hu">Jing Hu</name><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name><name sortKey="Yang, Yuanyuan" sort="Yang, Yuanyuan" uniqKey="Yang Y" first="Yuanyuan" last="Yang">Yuanyuan Yang</name><name sortKey="Zhang, Zijia" sort="Zhang, Zijia" uniqKey="Zhang Z" first="Zijia" last="Zhang">Zijia Zhang</name><name sortKey="Zhao, Lintao" sort="Zhao, Lintao" uniqKey="Zhao L" first="Lintao" last="Zhao">Lintao Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantImRecepV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000127 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000127 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PlantImRecepV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31988013
|texte= Investigation of the mechanism of action of Porana sinensis Hemsl. against gout arthritis using network pharmacology and experimental validation.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31988013" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PlantImRecepV1
| This area was generated with Dilib version V0.6.38. |  |